(2012-2019)

PUBLICATIONS 2019

Czerwiec Q, Idrissitaghki A, Imatoukene N, Nonus M, Thomasset B, Nicaud JM & Rossignol T (2019) Optimization of cyclopropane fatty acids production in Yarrowia lipolytica. Yeast.

Celińska E & Nicaud J-M (2019) Filamentous fungi-like secretory pathway strayed in a yeast system: peculiarities of Yarrowia lipolytica secretory pathway underlying its extraordinary performance. Appl Microbiol Biotechnol 103: 39-52.

Soudier P, Larroude M, Celinska E, Rossignol T & Nicaud JM (2019) Selection of Heterologous Protein-Producing Strains in Yarrowia lipolytica. Methods Mol Biol 1923: 153-168.

Celińska E, Borkowska M, Białas W, Kubiak M, Korpys P, Archacka M, Ledesma-Amaro R & Nicaud J-M (2019) Genetic engineering of Ehrlich pathway modulates production of higher alcohols in engineered Yarrowia lipolytica. FEMS Yeast Res 19: foy122-foy122.

Park Y-K, Korpys P, Kubiak M, Celinska E, Soudier P, Trébulle P, Larroude M, Rossignol T & Nicaud J-M (2019) Engineering the architecture of erythritol-inducible promoters for regulated and enhanced gene expression in Yarrowia lipolytica. FEMS Yeast Res 19: foy105-foy105.

PUBLICATIONS 2018 

Carly F, Steels S, Telek S, Vandermies M, Nicaud J-M & Fickers P (2018) Identification and characterization of EYD1, encoding an erythritol dehydrogenase in Yarrowia lipolytica and its application to bioconvert erythritol into erythrulose. Bioresour Technol 247: 963-969.

Celińska E & Nicaud J-M (2018) Filamentous fungi-like secretory pathway strayed in a yeast system: peculiarities of Yarrowia lipolytica secretory pathway underlying its extraordinary performance. Appl Microbiol Biotechnol.

Celińska E, Borkowska M, Białas W, Korpys P & Nicaud J-M (2018) Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags. Appl Microbiol Biotechnol 102: 5221-5233.

Larroude M, Rossignol T, Nicaud JM & Ledesma-Amaro R (2018) Synthetic biology tools for engineering Yarrowia lipolytica. Biotechnol Adv 36: 2150-2164.

Larroude M, Celinska E, Back A, Thomas S, Nicaud JM & Ledesma-Amaro R (2018) A synthetic biology approach to transform Yarrowia lipolytica into a competitive biotechnological producer of beta-carotene. Biotechnol Bioeng 115: 464-472.

Legrand M, Bachellier-Bassi S, Lee KK, et al. (2018) Generating genomic platforms to study Candida albicans pathogenesis. Nucleic Acids Res 46: 8664.

Leplat C, Nicaud JM & Rossignol T (2018) Overexpression screen reveals transcription factors involved in lipid accumulation in Yarrowia lipolytica. FEMS Yeast Res 18.

Niehus X, Crutz-Le Coq A-M, Sandoval G, Nicaud J-M & Ledesma-Amaro R (2018) Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials. Biotechnology for Biofuels 11: 11.

Park Y-K, Dulermo T, Ledesma-Amaro R & Nicaud J-M (2018) Optimization of odd chain fatty acid production by Yarrowia lipolytica. Biotechnology for Biofuels 11: 158.

Sagnak R, Cochot S, Molina-Jouve C, Nicaud J-M & Guillouet SE (2018) Modulation of the Glycerol Phosphate availability led to concomitant reduction in the citric acid excretion and increase in lipid content and yield in Yarrowia lipolytica. J Biotechnol 265: 40-45.

Vandermies M, Kar T, Carly F, Nicaud JM, Delvigne F & Fickers P (2018) Yarrowia lipolytica morphological mutant enables lasting in situ immobilization in bioreactor. Appl Microbiol Biotechnol.

PUBLICATIONS 2017

Bhutada G, Kavšcek M, Ledesma Amaro R, Thomas S, Rechberger GN, Nicaud J-M & Natter K (2017) Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica. FEMS Yeast Res 17.

Carly F, Gamboa Melendez H, Vandermies M, Damblon C, Nicaud J-M & Fickers P (2017) Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica (vol 101, pg 6587, 2017). Appl Microbiol Biotechnol 101: 6573-6573.

Carly F, Vandermies M, Telek S, Steels S, Thomas S, Nicaud J-M & Fickers P (2017) Enhancing erythritol productivity in Yarrowia lipolytica using metabolic engineering. Metab Eng 42: 19-24.

Celinska E, Ledesma Amaro R, Larroudé M, Rossignol T, Pauthenier C & Nicaud J-M (2017) Golden Gate Assembly system dedicated to complex pathway manipulation in Yarrowia lipolytica. Microbial Biotechnology 10: 450-455.

Dulermo R, Brunel F, Dulermo T, Ledesma Amaro R, Vion J, Trassaert M, Thomas S, Nicaud J-M & Leplat C (2017) Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica. Microbial Cell Factories 16: 1-11.

Gajdos P, Nicaud J-M & Certik M (2017) Glycerol conversion into a single cell oil by engineered Yarrowia lipolytica. Eng Life Sci 17: 325-332.

Guo Z, Duquesne S, Bozonnet S, Nicaud J-M, Marty A & O'Donohue M (2017) Expressing accessory proteins in cellulolytic Yarrowia lipolytica to improve the conversion yield of recalcitrant cellulose. Biotechnology for Biofuels 10: 16 p.

Guo Z, Duquesne S, Bozonnet S, Cioci G, Nicaud J-M, Marty A & O'Donohue M (2017) Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach. Biotechnology for Biofuels 10: 13.

Imatoukene N, Verbeke J, Beopoulos A, Taghki AI, Thomasset B, Sarde C-O, Nonus M & Nicaud J-M (2017) A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 101: 4605-4616.

Lazar Z, Neuvéglise C, Rossignol T, Devillers H, Morin N, Robak Mg, Nicaud J-M & Le Coq A-M (2017) Characterization of hexose transporters in Yarrowia lipolytica reveals new groups of Sugar Porters involved in yeast growth. Fungal Genet Biol 100: 1-12.

Park YK, Nicaud J-M & Ledesma-Amaro R (2017) The engineering potential of Rhodosporidium toruloides as a workhorse for biotechnological applications. Trends Biotechnol.

Trassaert M, Vandermies M, Carly F, Denies O, Thomas S, Fickers P & Nicaud J-M (2017) New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica. Microbial Cell Factories 16.

Trébulle P, Nicaud J-M, Leplat C & Elati M (2017) Inference and interrogation of a coregulatory network in the context of lipid accumulation in Yarrowia lipolytica. npj Systems Biology and Applications 3.

Vandermies M, Denies O, Nicaud J-M & Fickers P (2017) EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica. J Microbiol Methods 139: 161-164.

PUBLICATIONS 2016

Back A, Rossignol T, Krier F, Nicaud J-M & Dhulster P (2016) High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production. Microbial Cell Factories15: 147.

Dulermo R, Gamboa-Meléndez H, Ledesma‐Amaro R, Thevenieau F & Nicaud J-M (2016) Yarrowia lipolytica AAL genes are involved in peroxisomal fatty acid activation. Biochim Biophys Acta1861: 555-565.

Gajdoš P, Ledesma-Amaro R, Nicaud J-M, Čertík M & Rossignol T (2016) Overexpression of diacylglycerol acyltransferase in arrowia lipolytica affects lipid body size, number and distribution. FEMS Yeast Res16.

Ledesma-Amaro R & Nicaud J-M (2016) Metabolic Engineering for Expanding the Substrate Range of Yarrowia lipolytica. Trends Biotechnol34: 798-809.

Ledesma-Amaro R & Nicaud J-M (2016) Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids. Progress in Lipid Research61: 40-50.

Ledesma-Amaro R, Dulermo R, Niehus X & Nicaud J-M (2016) Combining metabolic engineering and process optimization to improve production and secretion of fatty acids. Metab Eng38: 38-46.

Ledesma-Amaro R, Lazar Z, Rakicka M, Guo Z, Fouchard F, Coq A-MC-L & Nicaud J-M (2016) Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose. Metab Eng38: 115-124.

Sassi H, Delvigne F, Kar T, Nicaud J-M, Coq A-MC-L, Steels S & Fickers P (2016) Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica. Microbial Cell Factories 15: 159.

PUBLICATIONS 2015

Dulermo R, Gamboa-Melendez H, Ledesma-Amaro R, Thevenieau F & Nicaud JM (2015) Unraveling fatty acid transport and activation mechanisms in Yarrowia lipolytica. Biochim Biophys Acta 1851: 1202-1217.

Dulermo R, Dulermo T, Gamboa-Melendez H, Thevenieau F & Nicaud JM (2015) Role of Pex11p in Lipid Homeostasis in Yarrowia lipolytica. Eukaryot Cell 14: 511-525.

Dulermo R, Gamboa-Melendez H, Michely S, Thevenieau F, Neuveglise C & Nicaud JM (2015) The evolution of Jen3 proteins and their role in dicarboxylic acid transport in Yarrowia. Microbiologyopen 4: 100-120.

Dulermo T, Lazar Z, Dulermo R, Rakicka M, Haddouche R & Nicaud JM (2015) Analysis of ATP-citrate lyase and malic enzyme mutants of Yarrowia lipolytica points out the importance of mannitol metabolism in fatty acid synthesis. Biochim Biophys Acta 1851: 1107-1117.

Gajdos P, Nicaud JM, Rossignol T & Certik M (2015) Single cell oil production on molasses by Yarrowia lipolytica strains overexpressing DGA2 in multicopy. Appl Microbiol Biotechnol [Epub ahead of print].

Leplat, C., Nicaud, J.-M., Rossignol, T., (2015). High-throughput transformation method for Yarrowia lipolytica mutant library screening.FEMS Yeast Res [Epub ahead of print]

PUBLICATIONS 2014

Beopoulos A, Verbeke J, Bordes F, Guicherd M, Bressy M, Marty A & Nicaud JM (2014) Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 98: 251-262.

Dulermo R, Gamboa-Melendez H, Dulermo T, Thevenieau F & Nicaud JM (2014) The fatty acid transport protein Fat1p is involved in the export of fatty acids from lipid bodies in Yarrowia lipolytica. FEMS Yeast Res 14: 883-896.

Duquesne S, Bozonnet S, Bordes F, Dumon C, Nicaud JM & Marty A (2014) Construction of a highly active xylanase displaying oleaginous yeast: comparison of anchoring systems. PLoS One 9: e95128.

Janbon, G., Ormerod, K.L., Paulet, D., Byrnes, E.J., 3rd, Yadav, V., Chatterjee, G., Mullapudi, N., Hon, C.C., Billmyre, R.B., Brunel, F., Bahn, Y.S., Chen, W., Chen, Y., Chow, E.W., Coppee, J.Y., Floyd-Averette, A., Gaillardin, C., Gerik, K.J., Goldberg, J., Gonzalez-Hilarion, S., Gujja, S., Hamlin, J.L., Hsueh, Y.P., Ianiri, G., Jones, S., Kodira, C.D., Kozubowski, L., Lam, W., Marra, M., Mesner, L.D., Mieczkowski, P.A., Moyrand, F., Nielsen, K., Proux, C., Rossignol, T., Schein, J.E., Sun, S., Wollschlaeger, C., Wood, I.A., Zeng, Q., Neuveglise, C., Newlon, C.S., Perfect, J.R., Lodge, J.K., Idnurm, A., Stajich, J.E., Kronstad, J.W., Sanyal, K., Heitman, J., Fraser, J.A., Cuomo, C.A., Dietrich, F.S., (2014). Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation. PLoS Genet. 10, e1004261.

Lazar Z, Dulermo T, Neuveglise C, Crutz-Le Coq AM & Nicaud JM (2014) Hexokinase-A limiting factor in lipid production from fructose in Yarrowia lipolytica. Metab Eng 26C: 89-99.

Maguire SL, Wang C, Holland LM, Brunel F, Neuveglise C, Nicaud JM, Zavrel M, White TC, Wolfe KH & Butler G (2014) Zinc finger transcription factors displaced SREBP proteins as the major Sterol regulators during Saccharomycotina evolution. PLoS Genet 10: e1004076.

Morin N, Calcas X, Devillers H, Durrens P, Sherman DJ, Nicaud JM & Neuveglise C (2014) Draft Genome Sequence of Rhodosporidium toruloides CECT1137, an Oleaginous Yeast of Biotechnological Interest. Genome Announc 2.

Morin, N., Crutz-Le Coq, A.-M., Rossignol, T., Nicaud, J.-M., (2014). Protocols for Monitoring Growth and Lipid Accumulation in Oleaginous Yeasts. In: T. J. McGenity (Ed.), Hydrocarbon and Lipid Microbiology Protocols, Humana Press, pp. 1-17.

PUBLICATIONS 2013

Dulermo T, Treton B, Beopoulos A, Kabran Gnankon AP, Haddouche R & Nicaud JM (2013) Characterization of the two intracellular lipases of Y. lipolytica encoded by TGL3 and TGL4 genes: new insights into the role of intracellular lipases and lipid body organisation. Biochim Biophys Acta 1831: 1486-1495.

Lazar Z, Rossignol T, Verbeke J, Crutz-Le Coq AM, Nicaud JM & Robak M (2013) Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications. J Ind Microbiol Biotechnol 40: 1273-1283.

Michely, S., Gaillardin, C., Nicaud, J. M. and Neuveglise, C. (2013). Comparative physiology of oleaginous species from the yarrowia clade. PLoS One 8, e63356. 

Verbeke, J., Beopoulos, A. and Nicaud, J. M. (2013). Efficient homologous recombination with short length flanking fragments in Ku70 deficient Yarrowia lipolytica strains. Biotechnol Lett 35, 571-6.

Papanikolaou S, Beopoulos A, Koletti A, Thevenieau F, Koutinas AA, Nicaud JM & Aggelis G (2013) Importance of the methyl-citrate cycle on glycerol metabolism in the yeast Yarrowia lipolytica. J Biotechnol 168: 303-314.

PUBLICATIONS 2012

Theerachat, M., Emond, S., Cambon, E., Bordes, F., Marty, A., Nicaud, J. M., Chulalaksananukul, W., Guieysse, D., Remaud-Simeon, M. and Morel, S. (2012). Engineering and production of laccase from Trametes versicolor in the yeast Yarrowia lipolytica. 
Bioresour Technol 125, 267-74. 

Gasmi, N., Lassoued, R., Ayed, A., Treton, B., Chevret, D., Nicaud, J. M. and Kallel, H. (2012). Production and characterization of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) expressed in the oleaginous yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 96, 89-101. 

Loira, N., Dulermo, T., Nicaud, J. M. and Sherman, D. J. (2012). A genome-scale metabolic model of the lipid-accumulating yeast Yarrowia lipolytica. BMC Syst Biol 6, 35. 

Nicaud, J. M. (2012). Yarrowia lipolytica. Yeast 29, 409-18.

Ogrydziak, D. M. and Nicaud, J. M. (2012). Characterization of Yarrowia lipolytica XPR2 multi-copy strains over-producing alkaline extracellular protease - a system for rapidly increasing secretory pathway cargo loads. FEMS Yeast Res 12, 938-48.

Beopoulos, A., Haddouche, R., Kabran, P., Dulermo, T., Chardot, T. and Nicaud, J. M. (2012). Identification and characterization of DGA2, an acyltransferase of the DGAT1 acyl-CoA:diacylglycerol acyltransferase family in the oleaginous yeast Yarrowia lipolytica. New insights into the storage lipid metabolism of oleaginous yeasts. Appl Microbiol Biotechnol 93, 1523-37. 

Duquesne, S., Bordes, F., Fudalej, F., Nicaud, J. M. and Marty, A. (2012). The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Enzymes. Methods Mol Biol 861, 301-12.

Gaillardin, C., Neuveglise, C., Kerscher, S. and Nicaud, J. M. (2012). Mitochondrial genomes of yeasts of the Yarrowia clade. FEMS Yeast Res 12, 317-31.

Kabran, P., Rossignol, T., Gaillardin, C., Nicaud, J. M. and Neuveglise, C. (2012). Alternative Splicing Regulates Targeting of Malate Dehydrogenase in Yarrowia lipolytica. DNA Res 19, 231-44.