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My main goal is to shed light on lipid dynamics in the plasma membrane of B. subtilis. Lipid dynamics has so far mostly been studied in eukaryotic membranes, where fast spectroscopy techniques like fluorescence correlation spectroscopy (FCS) have revealed that lipid diffusion in membranes is highly heterogeneous. It particularly revealed that certain lipid species transiently assemble and disassemble, a process thought to play a significant role for instance in cellular signalling. In comparison, fewer studies have been performed in prokaryotes and the dynamics of lipids in bacterial membranes remains widely unknown. My project therefore consists in using fluorescence microscopy in general and FCS in particular to measure the diffusion behaviour of the lipids contained in bacterial membranes.

1. Saha, D.; Schmidt, U.; Zhang, Q.; Barbotin, A.; Hu, Q.; Ji, N.; Booth, M. J.; Weigert, M.; Myers, E. W. Practical Sensorless Aberration Estimation for 3D Microscopy with Deep Learning. Opt. Express 2020, 28 (20), 29044. h

2. Barbotin, A.; Urbančič, I.; Galiani, S.; Eggeling, C.; Booth, M. Background Reduction in STED-FCS Using a Bivortex Phase Mask. ACS Photonics 2020, 7 (7), 1742–1753.

3. Tröger, J., Hoischen, C.; Perner, B.; Monajembashi, S.; Barbotin, A.; Löschberger, A.; Eggeling, C.; Kessels, M. M.; Qualmann, B.; Hemmerich, P. Comparison of Multiscale Imaging Methods for Brain Research. Cells, 2020, 25.

4. Antonello, J., Barbotin, A., Chong, E. Z., Rittscher, J. and Booth, M. J. Multi-scale sensorless adaptive optics: application to stimulated emission depletion microscopy. Optics Express 28, 16749 (2020).

5. Barbotin, A.; Urbančič, I.; Galiani, S.; Eggeling, C.; Booth, M.; Sezgin, E. Z-STED Imaging and Spectroscopy to Investigate Nanoscale Membrane Structure and Dynamics. Biophysical Journal 2020, 118 (10), 2448–2457.

6. Barbotin, A., Galiani, S., Urbancic, I., Eggeling, C., and Booth, M. J. (2019). Adaptive optics allows STED-FCS measurements in the cytoplasm of living cells. Optics express, 27(16), 23378-23395

2020-2021 (6 months): Postdoc in image analysis in the group of Marco Fritzsche. (University of Oxford, UK). Development of image analysis pipelines to monitor T-cell activation.

2016-2020: PhD, under the co-supervision of Martin Booth and Christian Eggeling ” (University of Oxford, UK). Thesis title: “Wavefront shaping for stimulated emission depletion microscopy.

2015-2016: Engineering degree in computational optics (Institut d’Optique d’Aquitaine, Bordeaux, France)

2012-2015: Engineering degree at Ecole polytechnique (Palaiseau, France).