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I joined the lab to work on an ANR project with the aim of investigating and understanding the recycling process of a lipid carrier, the undecaprenyl phosphate (C₅₅-P). This carrier allows the assembly of peptidoglycan by translocating the lipid II from the cytoplasm to the periplasm thanks to the flippase MurJ. However, while it has been previously established two classes of phosphatases take part in that process, the recycling of C₅₅-P is only partially known. Our goal is to determine the localisation of these membrane phosphatases in E. coli in order to understand the interplay between them and the peptidoglycan biosynthesis machineries by using recombinant fluorescent proteins and microscopy.

Experience:

2020-2021 : Study and development of thermosensitive inteins through the elaboration of a screening method in Escherichia coli – Institut Pasteur, Plasticity of the Bacterial Genome Unit

2019 : Investigate the sensitivity of different Candida species to a fungal secondary metabolite –Aberdeen University, Aberdeen Fungal Group

2017 : Construction of a recombinant strain of Bacillus subtilis expressing the listeriolysin O and a model of antigen, the chicken egg ovalbumin – University of Warsaw, Department of Applied Microbiology

Diplomas:

2019-2020: Master of Science in Molecular and Cellular Biology: Microbiology specialising in Molecular and Medical Bacteriology – Sorbonne Université

2017-2019: Bachelor of Science in Microbiology (Honours) – University of Aberdeen, Scotland

2015-2017: 2-year degree in Biological Engineering majoring in Biological and Biochemical Analyses – Université Paris-Est Créteil (UPEC)