and Hygiene of Materials (B2HM)
Responsable : Romain BRIANDET
RESEARCH PROJECT / SUBJECT
Every year in France, more than 50,000 people develop an infection related to food contaminated with pathogenic microorganisms. Nearly 400 were fatal. The main bacteria involved are now well known to the public: Salmonella, Listeria, E. coli EHEC, ... A recent survey invoked in 12% of these infections contamination of raw materials, and in 54% of cases of contamination from industrial surfaces. Indeed, these areas are supporting a vibrant ecosystem that may contain pathogenic microorganisms in biofilms. Biofilms are composed of spatially organized and stuck in a self-produced gel polymer matrix microorganisms. This organic cement can act as a "shield" in face to environmental stresses and antimicrobial action. Biofilms are a major factor in the persistence of pathogens in food workshops. To fight against these biocontaminants, manufacturers use a massive amount of chemical biocides. If the efficiency of these molecules is demonstrated on airborne bacteria (tests which are based on international standards bactericidal), this is far from the case in biofilms can be up to 1000 times more resistant to these treatments as their planktonic counterparts. Moreover, some of these chemicals are highly polluting to the environment. This has led Europe to adopt a new regulatory arsenal (European REACH biocide directive 98/8/EC) which will directly result in the prohibition of many antimicrobial chemicals, some of which are now widely used in the industry. Consumer safety. Environmental protection. Conflicting influences on the industry today lack objective information to guide their technology choices.
It is in this context of microbiological food safety that fits the research conducted in the Micalis-B2HM team (Bioadhesion-Biofilm and hygiene of materials). Our work is focused on understanding the dynamic regulatory mechanisms of microbial adhesion to surfaces and biofilm formation. Are considered cell physiology, antimicrobial resistance, environmental conditions and their variations. The work developed contribute to the identification of situations conducive to the development of microbiological hazards, and aim to provide innovative management actions both preventive (anti-adhesive surfaces, positive biofilms .... ) and curative (chemical or biological treatment). The approaches developed are transferable to other areas of applications in collaboration, especially in the medical sector (~ 7% of patients acquire a nosocomial infection during a hospital stay, 65% of these infections are directly related to the presence of biofilms ...).
Several disciplines are intertwined in the team to address this issue ie microbiology, physical chemistry, microscopy and more recently molecular biology. It requires knowledge of the interfacial properties as support materials as biological surfaces. It uses various techniques of characterization, molecular analysis and observation of the (bio) surfaces, but also to the understanding of physical phenomena at interfaces (solid-liquid-air).
The B2HM team is located on the INRA de Jouy-en-Josas campus :
- Adress UMR1319 MICALIS, team B2HM, INRA-AgroParisTech, Domaine de Vilvert 78 350 Jouy-en-Josas
- E-mail email@example.com
- Téléphone 00 (33) 1 74 07 16 98