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INRAE

24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Stephen McGovern

AI INRAE

Stephen McGOVERN
Position : Assistant-Engineer
In the Lab Since : 15/01/1992
Email : stephen.mc-govern[at]inrae.fr
Tél : 01 34 65 25 08

Project Description

I implement, within the team, a methodolgical competence in molecular biology, genetics and protein biochemistry, most notably in projects aiming to characterise, in vivo as well as in vitro, the composition and functioning of nucleo-protein complexes relating to transcription regulation and DNA metabolism.
My activities more particulary consist in purifying interesting proteins and characterising their properties: researching of interactions with other proteins (through molecular sifting) or with DNA substrates (delaying gels), quantification of the in vivo expression of these proteins (Western-blot), capture of protein complexes (TAP-tag technique).

Publications

1. Sak4 of phage HK620 is a RecA remote homolog with single-strand annealing activity stimulated by its cognate SSB protein. Geoffrey Hutinet, Arthur Besle, Olivier Son, Stephen McGovern, Raphaël Guerois, Marie-Agnès Petit*, Françoise Ochsenbein and François Lecointe*. Front. Microbiol., 24 April 2018

2. Molecular and Physiological Logics of the Pyruvate-Induced Response of a Novel Transporter in Bacillus subtilis. Teddy Charbonnier, Dominique Le Coq, Stephen McGovern, Magali Calabre, Olivier Delumeau, Stéphane Aymerich, Matthieu Jules. mBio. 00976-17 3 October 2017

3. Multiple and Variable NHEJ-Like Genes Are Involved in Resistance to DNA Damage in Streptomyces ambofaciens. Hoff G, Bertrand C, Zhang L, Piotrowski E, Chipot L, Bontemps C, Confalonieri F, McGovern S, Lecointe F, Thibessard A, Leblond P. Front Microbiol. 2016 Nov 28;7:1901

4. C-terminal region of bacterial Ku controls DNA bridging, DNA threading and recruitment of DNA ligase D for double strand breaks repair. McGovern S, Baconnais S, Roblin P, Nicolas P, Drevet P, Simonson H, Piétrement O, Charbonnier JB, Le Cam E, Noirot P, Lecointe F. Nucleic Acids Res. 2016 Mar 9.

5. The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks. Costes A, Lecointe F, McGovern S, Quevillon-Cheruel S, Polard P. PLoS Genet. 2010 Dec 9.

6. The lactococcal abortive infection protein AbiP is membrane-anchored and binds nucleic acids. Domingues S, McGovern S, Plochocka D, Santos MA, Ehrlich SD, Polard P, Chopin MC. Virology. 2008 Mar 30

7. Control of methionine synthesis and uptake by MetR and homocysteine inStreptococcus mutans. Sperandio B, McGovern S, Ehrlich DS, Renault P, Martin-Verstraete I, Guédon E. J Bacteriol. 2007 Oct;1

8. Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks. Lecointe F., Sérèna C, Velten M, Costes A, McGovern S, Meile JC, Errington J, Ehrlich SD, Noirot P, Polard P. EMBO J. 2007 Oct 3

9. A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA. Mortier-Barrière I, Velten M, Dupaigne P, Mirouze N, Piétrement O, McGovern S, Fichant G, Martin B, Noirot P, Le Cam E, Polard P, Claverys JP. Cell. 2007 Sep 7

10. A distinct single-stranded DNA-binding protein encoded by the Lactococcus lactis bacteriophage bIL67. Agnieszka K. Szczepanska, Elena Bidnenko, Danuta Płochocka, Stephen McGovern, S. Dusko Ehrlich, Jacek Bardowski, Patrice Polard and Marie-Christine Chopin. Virology 2007 Feb 19.

11. Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus subtilis. Marie-Françoise Noirot-Gros, M. Velten, M. Yoshimura, S. McGovern, T. Morimoto, S. D. Ehrlich, N. Ogasawara, P. Polard, and Philippe Noirot (2006) Proc. Natl. Acad. Sci. Feb 14

12. Functional interplay between the Bacillus subtilis DnaD and DnaB proteins essential for initiation and re-initiation of DNA replication. Claude Bruand, Marion Velten, Stephen McGovern, Stéphanie Marsin, Céline Sérèna, S. Dusko Ehrlich and Patrice Polard (2005) Mol Microbiol. Feb.

13. The bacterial condensin/cohesin-like protein complex acts in DNA repair and regulation of gene expression. Etienne Dervyn, Marie-Françoise Noirot-Gros, Peggy Mervelet, Steven McGovern, S. Dusko Ehrlich, Patrice Polard and Philippe Noirot (2004) Mol Microbiol. Mar

14. A two-protein strategy for the functional loading of a cellular replicative DNA helicase. M. Velten, S. McGovern, S. Marsin, S. Ehrlich, P. Noirot, P. Polard (2003) Mol Cell. Apr;11

15. Restart of DNA replication in Gram-positive bacteria: functional characterisation of the Bacillus subtilis PriA initiator. Patrice Polard, Stéphanie Marsin, Stephen McGovern, Marion Velten, Dale B. Wigley, S. Dusko Ehrlich and Claude Bruand (2002) Nucleic Acids Res. Apr 1

16. DnaB, DnaD and DnaI proteins are components of the Bacillus subtilis replication restart primosome. Claude Bruand, Michaël Farache, Stephen McGovern, S. Dusko Ehrlich, Patrice Polard (2001) Mol Microbiol. Oct

17. Early steps of Bacillus subtilis primosome assembly. Stéphanie Marsin, Stephen McGovern, S. Dusko Ehrlich, Claude Bruand, and Patrice Polard (2001) J Biol Chem. Dec 7

18. PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication. Marie-Agnès Petit, Etienne Dervyn, Matthias Rose, Karl-Dieter Entian, Steven McGovern, S. Dusko Ehrlich & Claude Bruand (1998) Mol Microbiol. Jul;29

19. Replication terminus for DNA polymerase I during initiation of pAM beta 1 replication: role of the plasmid-encoded resolution system. Jannière L., Bidnenko V., McGovern S., Ehrlich SD., Petit MA. (1997) Mol Microbiol. Feb;23

20. In vivo analysis of the plasmid pAM beta 1 resolution system.
Laurent Jannière, Steven McGovern , Catherine Pujol, Marie-Agnès Petit and S. Dusko Ehrlich (1996) Nucleic Acids Res.

Curriculum Vitae

1988 DUT in Biological and Biochemical Analyses.
1989-1991 Quality control technicial in the pharmaceutical industry.
1992 Recruited at INRA in Jouy-en-Josas as Research Technician
2007 Promoted to Assistant-Engineer.