Bacterial Cell Surfaces, Gordon Research Conference
Human Microbiome: From Models and Mechanism to Medicine
Claire-Jing Rouchet – 27/11/2024
The effect of membrane fluidity on cell wall shape and elongation in Bacillus subtilis
A method for the quantification of membrane fluidity in bacteria
Membrane fluidity is a critical element in bacterial phenotype: it is the parameter that governs the rate of diffusion of membrane proteins. The fluidity of a membrane is modulated by the physical parameters of its environment, first and foremost by temperature: when the temperature drops, so does the fluidity of a membrane. In response to […]
Organisation of MreB protein protofilaments in Gram-positive bacteria
First observation by electron microscopy of pairs of organised protofilaments and first three-dimensional structure of the bacterial homologue of MreB actin from a Gram-positive bacterium Research into eukaryotic actin has historically focused on elucidating structure-function relationships from in vitro studies. However, purification of the prokaryotic actin MreB has proved particularly difficult, thwarting efforts to carry […]
Deciphering the polymerization cycle of a bacterial actin-like protein
From eukaryotic to bacterial cells, cytoskeletal proteins play critical roles in various aspect of cell physiology, including cell shape and cytokinesis, chromosomes partitioning, and intracellular transport. In bacteria, MreB, an homolog of eukaryotic actin, is part of the “Rod complex”, a group of essential protein that synthesize the cell wall (CW), the extracellular shell that […]