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My work focuses on the role played by protein-protein interactions (PPI) - as well as their dynamics - in cellular processes. These PPI have been shown for a long time to be crucial in various cellular processes such as enzyme activities, signal transduction or "ad hoc" gene transcription. Unraveling this network of PPIs is therefore important to understand how cellular processes work and are coordinated in the cell.
From the end of 2006 to April 2011, I participated in the European project BaSysBio which has used a Systems Biology approach in Bacillus subtilis, through a study on the transcription machinery (see publications below) and some transcription factors. Our aim was to show the protein-protein interactions involved in transcription regulation, as well as their potential involvement in transcriptional regulation networks. These protein-protein interactions were detected or validated either by using the Yeast Two-Hybrid System, or with the in vivo TAP Tag technology associated with mass spectrometry or even in vitro after purification of the proteins expressed in E. coli. This project is currently being finalized, with some last experiments and writing of publications (this work was done in collaboration with Tatiana Rochat, Etienne Dervyn and François Lecointe)
I am currently starting a project in protein-protein interactions, this time involving membrane and/or peripheral or even cell wall proteins in Bacillus subtilis. Although located at the strategic interface between the inside and outside of the bacterium, protein-protein interactions in this compartment have hardly been explored, for the simple reason that it represents an important technical challenge.

- DELUMEAU O., LECOINTE F., MUNTEL J., GUILLOT A., GUÉDON E., MONNET V., HECKER M., BECHER D., POLARD P., NOIROT P. (2011) The dynamic protein partnership of RNA polymerase inBacillus subtilis. Proteomics. 11:2992-3001
- MARLES-WRIGHT J., GRANT T., DELUMEAU O., VAN DUINEN G., FIRBANK S.J., LEWIS P.J., MURRAY J.W., NEWMAN J.A., QUIN M.B., RACE P.R., ROHOU A., TICHELAAR W., VAN HEEL M., LEWIS R.J. (2008) Molecular architecture of the "stressosome," a signal integration and transduction hub. Science, 322(5898):92-96.
- HARDWICK S.W., PANE-FARRE J., DELUMEAU O. MARLES-WRIGHT J., MURRAY JW, HECKER M., and LEWIS RJ. (2007) Structural functional characterisation of partner-switching regulating the environmental stress response in B. subtilis. Journal of Biological Chemistry, 282: 11562-72.
- DELUMEAU O., CHEN C-C., MURRAY J.W., YUDKIN M.D., and LEWIS R.J. (2006) High-Molecular-Weight Complexes of RsbR and Paralogues in the Environmental Signaling Pathway of Bacillus subtilis. Journal of Bacteriology, 188: 7885-7892.
- MURRAY JW., DELUMEAU O., and LEWIS R.J. (2005) Structure of a nonheme globin in environmental stress signaling. Proc Natl Acad Sci U S A. 102: 17320-17325.
- CHEN C-C., YUDKIN M.D., and DELUMEAU O. (2004) Phosphorylation and RsbX-dependent dephosphorylation of RsbR in the RsbR:RsbS complex of Bacillus subtilis. Journal of Bacteriology, 186: 6830-6836.
- DELUMEAU O, DUTTA S, HARDWICK SW, VOLKER U, YUDKIN M.D. and LEWIS R.J. (2004) Functional and Structural Characterization of RsbU, a Stress Signaling Protein Phosphatase 2C. Journal of Biological Chemistry, 279, 40927-40937.
- CHEN C-C., HARRIS R., LEWIS R. J. YUDKIN M.D and DELUMEAU O. (2003) A supramolecular complex in the environmental stress signalling pathway of Bacillus subtilis. Molecular Microbiology. 49, 1657-1669.
- ROLLENHAGEN C., ANTELMANN H., KIRSTEIN J., DELUMEAU O., HECKER M. and YUDKIN M.D. (2003) Binding of A and B to core RNA polymerase after environmental stress in Bacillus subtilis. Journal of Bacteriology, 185, 35-40.
- DELUMEAU O., LEWIS R.J and YUDKIN M.D. (2002) Protein-protein interactions that regulate the energy stress activation of B in Bacillus subtilis. Journal of Bacteriology, 184, 5583-5589.
Thèse
- DELUMEAU O., RENARD M. and F. MONTRICHARD (2000) Characterization and possible redox regulation of the purified calmodulin-dependent NAD+ kinase from Lycopersicum pimpinellifolium. Plant, Cell and Environment, 23, 1267-1273.
- DELUMEAU O., MORERE-LEPAVEN M-C., MONTRICHARD F. and D.L. LAVAL-MARTIN (2000) Effects of short-term NaCl stress on calmodulin trancript levels and calmodulin-dependent NAD kinase activity in two species of tomato. Plant, Cell and Environment, 23, 329-336.
- DELUMEAU O., MONTRICHARD F. and D.L. LAVAL-MARTIN (1998) NAD+ kinase activity, calmodulin levels during the growth of isolated cells from Lycopersicon pimpinellifolium and kinetic constants of the calmodulin-dependent NAD+ kinase. Plant Science, 138, 43-52.

• PhD on the interaction between NAD kinase and calmodulin in tomato at Angers University, France (1995-1998), then ATER (1998-1999)
• Research Associate at the Universities of Oxford (2000-2003) and Newcastle (2003-2006), on the regulation of the sigmaB factor in Bacillus subtilis.
• At INRA in Jouy-en-Josas since November 2006 for the beginning of the BaSysBio Project.
• Permanent position as Research Associate in Philippe Noirot's lab since September 2008