Know more

The goal of my research project is to decipher the rules driving gene expression in the gram positive bacterium B. subtilis. This project will complete our knowledge of the biology of B. subtilis and will allow future rational designs of genetic circuits dedicated to biotechnological and synthetic biology applications. To summarize the project, we are interested in building and characterizing a library of synthetic genetic modules. These modules give us the ability to control transcription and translation of a gene of interest in the bacterium B. subtilis. The library of genetic sequences is used to control the production of a fluorescent protein. Its production level is analyzed during B. subtilis growth in various growth media (as a function of the medium richness).

Articles

[1] Kandel S., Sauveplane V., Compagnon V., Franke R., Millet Y., Schreiber L., Werck-Reichhart D. & Pinot F (2007) Characterization of a methyl jasmonate and wounding-responsive cytochrome P450 of Arabidopsis thaliana catalyzing dicarboxylic fatty acid formation in vitro. FEBS J 274, 5116-5127

[2] Ehlting J., Sauveplane V., Olry A., Ginglinger J.F., Provart N.J. & Werck-Reichhart D (2008) An extensive (co-)expression analysis tool for the cytochrome P450 superfamily in Arabidopsis thaliana. BMC Plant Biol 8, 47

[3] Serra O., Soler M., Hohn C., Sauveplane V., Pinot F., Franke R., Schreiber L., Prat S., Molinas M., Figueras M. (2008) CYP86A33 targeted gene silencing in potato tuber alters suberin composition, distorts suberin lamellae and impairs the periderm's water barrier function. Plant Physiol 149(2), 1050-60

[4] Sauveplane V., Kandel S., Kastner P.E., Ehlting J., Compagnon V., Werck-Reichhart D. & Pinot F (2009) Arabidopsis thaliana CYP77A4 is the first cytochrome P450 able to catalyze epoxidation of free fatty acids in plants. FEBS J 276(3), 719-35

[5] Beisson-Li Y., Pollard M., Sauveplane V., Pinot F., Ohlrogge J., & Beisson F (2009) Nanoridges that characterize the surface morphology of flowers require the synthesis of cutin polyester. PNAS 106(51), 22008-13

[6] Li H., Pinot F., Sauveplane V., Werck-Reichhart D., Diehl P., Schreiber L., Franke R., Zhang P., Chen L., Gao Y., Liang W., & Zhang D (2010) Cytochrome P450 Family Member CYP704B2 Catalyzes the ω-Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice. Plant Cell 22(1), 173-90

[7] Kastner P.E., Le Calvé S., Diss L., Sauveplane V., Franke R., Schreiber L., & Pinot F (2011) Specific accumulation of CYP94A1 transcripts after exposure to gaseous benzaldehyde: induction of lauric acid ω-hydroxylase activity in Vicia sativa exposed to atmospheric pollutants. Environ Res 111(1), 37-44

[8] Luque A, Sebai SC, Santiago-Schübel B, Le Coz Y, Jenot D, Ramaen O, Sauveplane V & Pandjaitan R (2014) In vivo evolution of metabolic pathways by homeologous recombination in mitotic cells. Metab Eng 23, 123–135

[9] Luque A, Sebai SC, Sauveplane V, Ramaen O & Pandjaitan R (2014) In vivo evolution of metabolic pathways: Assembling old parts to build novel and functional structures. Bioengineered, 1–10

[10] Guiziou S, Sauveplane V, Chang H-J, Cler E C, Declerck N, Jules M & Bonnet J (2016) A part toolbox to tune genetic expression in Bacillus subtilis. Nucleic Acids Res. 44, 7495–7508

▪ Reviews

[11] Kandel S., Sauveplane V., Olry A., Diss L., Benveniste I. & Pinot F (2006) Cytochrome P450-dependent fatty acid hydroxylases in plants. Phytochem Rev 5, 359-372

[12] Sauveplane V., Olry A., Kandel S., Werck-Reichhart D. & Pinot F (2006) Expression of plant cytochrome P450-dependent fatty acid hydroxylases in insect cells. In: Current Advances in the Biochemistry and Cell Biology of plant Lipids 2006 (Christoph Benning and John Ohlrogge eds) pp 185-189 Aardvark Global Publishing Company, LLC, Salt lake City, UT, USA

[13] Fickers P., Sauveplane V., & Nicaud J-M (2013) The lipases from Y. lipolytica: genetics, production, regulation and biochemical characterization. Microbiology Monographs, In Biology and Biotechnology of Yarrowia lipolytica Biotechnological Applications, Barth G. editor. Springer-Verlag Berlin Heidelberg. 25, 99-120

▪ Patents

[14] Sauveplane V., Ehlting J., Werck-Reichhart D. & Pinot F (2008) Expression cassettes for seed-enhanced expression in plants. EP08356144.9 and US61/201654

[15] Ramaen O., Sauveplane V. & Pandjaitan R (2013) Recombinant host cell for biosynthetic production. EP2013078117

[16] Ramaen O., Sauveplane V., Pandjaitan R & Gelo-Pujic M (2014) Production améliorée de vanilloïdes par fermentation. EP14305939.2

[17] Nicaud J.M., Thevenieau F, Sauveplane V., Gamboa-Melendez H. & Morin N (2015) Microorganisms and use thereof for the production of diacids. WO2016EP56693